Warning Cookies are used on this site to provide the best user experience. If you continue, we assume that you agree to receive cookies from this site. OK

DDS catalog request

6. SS-cleavable and pH-responsive lipid like material (COATSOME® SS-Series) !NEW!

SS-cleavable and pH-responsive Lipid Like Material (COATSOME® SS-Series)

COATSOME® SS-series New

SS-cleavable and pH-responsive lipid-like material for gene delivery and nucleic acid delivery system

We have developed COATSOME® SS-series lipids for effective intracellular delivery and lower toxicity. COATSOME® SS-series contains dual sensing motifs that can respond to the intracellular environment; tertiary amines respond to an acidic compartment (endosome/lysosome) for membrane destabilization, and disulfide bond that can cleave in reductive environment (cytoplasm). These two units synergize for efficient intracellular delivery of encapsulated drugs with enhanced biosafety.

SSLipids1

SS-OP2

*Products of above table are available online. Please check the bottom of this page.

• Proof of concept

Particle formation

Serum resistance

Particle disruption in the cytoplasm

Using the non-cleavable lipid as a component of membrane, pDNA dominantly colocalizes with lipid membrane. In contrast, using SS-Cleavable lipid instead of the non-cleavable lipid, the pDNA does not colocalize with membrane and can be found prominently. These show that cleavage of the disulfide bonding accelerates particle disruption.

• Toxitity of the particles

Cytotoxicity of the particles

Toxicity of the particles in vivo

SS lipids toxicity in vivo

In vitro transfection applying non-cleavable lipid shows sharp decline of cell survival rate by increase of the dose concentration. On the other hand, applying SS-Cleavable lipid, high cell survival rate is kept even when the dose is increased up to 10 folds of general transfection condition. Further, in the hepatotoxicity marker, SS-Cleavable lipid clearly shows biological safety in comparison with non-cleavable lipid.