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Ultrapure Polysorbate 80

Polysorbate 80 (HX2)™

Polyoxyethylene Sorbitan Oleate

Polysorbate 80(HX2)™ produced by NOF CORPORATION is a ultrapure product of the highest quality in the world, with extremely low aldehyde and peroxide levels.

These characteristics contribute to its safety; in a rat study, Polysorbate 80(HX2)™, as compared to conventional Polysorbate 80 formulations, triggered less histamine release from rat mast cells. The latest in vivo dog study has also indicated less histamine release with our Polysorbate 80(HX2); as a natural consequence, because of the lower incidence of allergy noted, our Polysorbate 80(HX2)™ has drawn worldwide attention.

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Features of Polysorbate 80 (HX2)™

  • Low Allergic Reaction (Low Degranulation)
  • High Safety Features (Low Cell Toxicity & Hemolysis)
  • High Stability
  • Low Impurities
  • Low Peroxide
    • Vegetable Source (No animal sources)
    • Colorless and Odorless

    Degranulation Test (An allergic study model)

    Effect of Polysorbate 80 on degranulation of RBL-2H3 mast cells. Cells were treated with different concentrations of Polysorbate 80 for 60 mins. The degree of degranulation was determined by measurement of the released ß-hexosaminidase into the supernatant.

    Influence on Basophillic leukocyte

    Basophilic leukocytes were immersed in 1% Polysorbate 80 solution for 30 min. The cells were immobilized in glutaraldehyde and observed by SEM.

    Cell Toxicity Test

    Effect of Polysorbate 80 concentration on the cytotoxicity using SIRC Cells. Cells were treated with each Polysorbate 80 for 24 hrs. The number of viable cells was determined by the Neutral Red Uptake method.

    Hemolysis Test

    Effect of Polysorbate 80 concentration on the Hemolytic Ratio. Red blood cells from the guinea pig were treated with Polysorbate 80 for 60 mins. The hemolytic ratio was determined by the absorbance of the solution at 576 nm.

    Survival Rate

    Effect of Polysorbate 80 concentration on the Survival Rate of BALB/c mice. Polysorbate 80 was diluted with PBS and administered to mice by intravenous injection.

    Stability Test

    This formaldehyde test was performed under air sealed conditions at 40°C.

    Ultra-Purity Polysorbate

    Polysorbate 80 (HX2)™

    Polysorbate 80(HX2) GMP Grade

    Ultra Purity Polysorbate 80, GMP Grade
    Chemical name: Polyoxyethylene sorbitan monooleate
    CAS#: 9005-65-6
    Specification: JP, EP, USP/NF, ChP
    Package size: 1kg / 10kg

    Polysorbate 80(HX2) non-GMP Grade

    Ultra Purity Polysorbate 80, non-GMP Grade
    Chemical name: Polyoxyethylene sorbitan monooleate
    CAS#: 9005-65-6
    Specification: JP, EP, USP/NF
    Package size: 100g