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Biocompatible Coating Polymer

Biocompatible Coating Polymers

Lipidure®-CM

Biocompatible coating polymer suppresses cell and protein adsorption on the surface. Lipidure®-CM coated surface is covered with phosphorylcholine units. Any cells and proteins do not adhere to the surface. Lipidure® can be coated on plastics such as PET, PMMA, PS (PSt), PU, PC, PE, and PP, glass, ceramics, and metals such as SUS, titanium, and gold.


Coating Method of biocompatible coating polymer


PC surface can be made by coating with Lipidure®-CM. The following figure shows the relationship between “the number of times the coating was applied” and “the thickness of the coating film”. The thickness of the Lipidure®-CM coating film is calculated by weight measurement. These data are examples in the case of dip coating. The thickness was controlled by adjusting the number of times of a coating was applied and the concentration of Lipidure® in the coating solution.

Lipidure-CM5206 can be coated on the following materials:

  • Glass
  • Plastic (PET, PMMA, PSt, PU, PC, PE, PP)
  • Metal (SUS, Titanium, Gold)
  • Ceramic

Feature of biocompatible coating polymer

  • Suppression of cell and protein absorption
  • Suppression of cell activation
  • Suppression of protein denature
  • Hemo-compatibility

Suppression of protein adsorption

Method

  1. Prepare well plate coated with Lipidure®-CM5206
  2. Add HRP-IgG conjugate solution into each well
  3. Incubate for 1 hr at 25 ºC
  4. Rinse three times with 0.05 % Tween20/Dulbeco’s PBS
  5. Add of 3,3’,5,5’-tetramethylbenzidine solution into each well
  6. Measure adsorbance at 450 nm

Lipidure®-CM5206 suppresses protein adsorption significantly

Suppression of cell adsorption

Method

  1. Prepare well plate coated with Lipidure®-CM5206
  2. Seed macrophage in well plate at 3.3x105 cells/well
  3. Incubate for 1 hr at 37 ºC in 5% CO2 atmosphere
  4. Rinse off with Dulbeco’s PBS
  5. Fix with glutaraldehyde and Observation by SEM

Lipidure®-CM5206 prevents macrophage from adhesion

Hemo-compatibility

Method

  1. Prepare artificial vascular coated with Lipidure®-CM5206
  2. Rabbit blood was flowed in Lipidure®-CM5206 treated artificial vascular
  3. Rinse artificial vascular with buffer solution
  4. Take photo of inside of artificial vascular

Lipidure®-CM5206 prevents macrophage from adhesion


Lipidure®-CM

Lipidure®-CM

Lipidure®-CM5206

Coating Reagents Lipidure® CM5206
Synthetic lipid polymer for biocompatible surface

Usage
-Dissolve Lipidure®-CM in ethanol and prepare a 0.5wt% ethanol solution.
-Dip the base material into the coating solution for 1 min.
-Dry the coated material at room temperature or one hour at 50°C.

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Lipidure®-CM5206 (10g)

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Usage
-Dissolve Lipidure®-CM in ethanol and prepare a 0.5wt% ethanol solution.
-Dip the base material into the coating solution for 1 min.
-Dry the coated material at room temperature or one hour at 50°C.

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Lipidure®-NH

Lipidure®-NH

Lipidure-NH01

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Lipidure®-CR

Lipidure®-CR

Lipidure-CR2001

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